Table 2 Summary of the microsatellite loci and PCR primers developed for the flea Xenopsylla cheopis
From: Genetic structure and gene flow of the flea Xenopsylla cheopis in Madagascar and Mayotte
Multiplex pool | Primer (locus) names | Primer sequences (5′–3′) | Repeat motifs | Size range (bp) | Allele number |
|---|---|---|---|---|---|
Pool 1 | XC024 | F: 6-FAM-ATGCAGCTCGTTCGTCTCC | CA(5)...CA(13)...CA(5) | ind | – |
R: GTCCAATTCATCCGCATCG | |||||
XC009 | F: NED-CATTGCGGGAGCATCAG | (CA)5...(CA)8 | 283–303 | 6 | |
R: TGCAGGCACAAAATTCGAC | |||||
XC018 | F: PET-TCGATTCAGCCGTTTTCG | (CA)10 | ind | – | |
R: TTGGAGAAGGAGATGTGTATGC | |||||
XC037 | F: VIC-GGGCCACCGAGTTGACG | [(GTT)2...]13 | 290–348 | 4 | |
R: TGGTGTTCCGTTACCGTTCC | |||||
Pool 2 | XC007 | F: 6-FAM-CTGGTTGGATTGTCTCC | (CA)5...(CA)22 | ind | – |
R: ACTATGCCGGATTAAGG | |||||
XC021 | F: PET-AGTGGACCGAGAACAGAGC | (GT)9 | 242–258 | 5 | |
R: TCATGTAAAGAGACCTGAGACC | |||||
SF009 | F: NED-CGTGTAGTTGCGAGAGAAGC | (GT)4...(GT)5 | 168–187 | 5 | |
R: GGAGAAGTGCGTTTACAGAGC | |||||
XC013 | F: VIC-CAAAATTGGAGAAGGAGACG | [GT(2)..GTAT]5 | 164–235 | 5 | |
R: AAATCGTTGACGGAAGAAGC | |||||
Pool 3 | XC023 | F: PET-CTAGTAAACGCAAACGCTACC | [GT(3)...]7 | 300–380 | 5 |
R: CCCCCAAACAAATCAGC | |||||
XC044 | F: 6-FAM-AAAAGTAAAGTCGAACAAGTGG | (GTAT)6...(GT)5...(GTAT)8 | 396–428 | 5 | |
R: GCTTATAGGTTACAAACATCTGG | |||||
XC016 | F: NED-ATCGACCCCAAAATCAGC | GT(10)...GTAT(12)...GT(7) | 370–400 | 11 | |
R: ACCCCTGGTTGGATTGC | |||||
XC002 | F: VIC-GCAGGCACAAAATTCGACA | GT(9)...GT(5) | 266–302 | 5 | |
R: GCGGGGGCATCAGTTAAT |