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Fig. 5 | Parasites & Vectors

Fig. 5

From: Identification of Anaplasma ovis appendage-associated protein (AAAP) for development of an indirect ELISA and its application

Fig. 5

Identification of native AAAP form the different weight lysate of A. ovis in Western blot. Lane M: molecular weight marker; Lanes 1–8: blotted with A. ovis lysate (Lanes 1 and 5: 20 μg; Lanes 2 and 6, 15 μg; Lanes 3 and 7, 10 μg; Lanes 4 and 8, 5 μg); Lanes 9–10: blotted with recombinant AAAP, reacted with rabbit anti-rP35 antibody; Lanes 1–4, 10, reacted with rabbit anti-rAAAP antibody; Lanes 5–9, reacted with pre-immunized rabbit serum

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