Fig. 6From: Identification of Anaplasma ovis appendage-associated protein (AAAP) for development of an indirect ELISA and its applicationDetermination of ELISA cut-off value. The cut-off value of the rAAAP ELISA was determined to be 6.0% by testing 434 negative and 163 positive reference sera using interactive dot diagram. With this threshold, the calculated sensitivity and specificity were 95.1 and 95.4%, respectivelyBack to article page