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Fig. 4 | Parasites & Vectors

Fig. 4

From: A case study of Desmozoon lepeophtherii infection in farmed Atlantic salmon associated with gill disease, peritonitis, intestinal infection, stunted growth, and increased mortality

Fig. 4

Abdominal cavity with spleen and severe D. lepeophtherii infection in both a diffuse acute peritonitis and chronic vaccine related lesions. a HE-stain, spleen (*) and intestine (arrow) and perivisceral inflammatory tissue (white bar). b-f show details of a: b HE-stain of perivisceral inflammatory tissue with necrosis and iridescent spores (*) and pink proliferative stages of D. lepeophtherii surrounded by a halo (arrows). c ISH of same area as b showing proliferative stages of D. lepeophtherii stained red (arrow) including in a necrotic cell (*). d ISH staining proliferative stages of D. lepeophtherii red in the border area of spleen and perivisceral inflammatory tissue, many in the peritonitis, a few in the spleen, arrowhead pointing at the spleen capsule. e ISH staining proliferative stages of D. lepeophtherii red both in the diffuse peritonitis and in the organized, circular encapsulation of a droplet (*), presumably vaccine in oil-adjuvant. f FCP of border area of spleen and perivisceral inflammatory tissue showing green fluorescent D. lepeophtherii spores (arrow) especially in the peritonitis, while the spleen (*) appear less infected borderline between spleen and peritoneum (arrowhead). Scale-bars: a, 1 mm; b, c, 10 μm; d-f, 50 μm

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Parasites & Vectors

ISSN: 1756-3305

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