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Table 1 Summary of the R. zambeziensis transcriptome assembly and annotation statistics

From: Sialotranscriptomics of Rhipicephalus zambeziensis reveals intricate expression profiles of secretory proteins and suggests tight temporal transcriptional regulation during blood-feeding

Transcriptome statistics Valuea
Transcriptome assembly statistics
 Total number of transcripts 23,631
 Number of transcripts > 500 bp 19,903
 Number of transcripts > 1 kb 13,330
 Number of transcripts > 10 kb 80
 Shortest transcript length (bp) 201
 Longest transcript length (bp) 17,108
 Mean length of transcripts (bp) 1793.5
 Median length of transcripts (bp) 1193
 Transcript N50 (bp) 2807
 Total bases in assembly (Mb) 42.4
 Ambiguous base calls (Ns) 0
 GC content (%) 49
 Number of non-redundant predicted proteins 13,584
Transcriptome annotation statisticsb
 BLASTx against NR 12,756 (54.0%)
 BLASTx against UniProtKB/TrEMBL 16,451 (69.6%)
 BLASTx against UniProtKB/Swiss-Prot 10,572 (44.7%)
 BLASTx against TSA-NR 16,711 (70.7%)
 BLASTx against Ixodes scapularis predicted peptides 11,804 (50.0%)
 BLASTx against EuKaryotic Orthologous Groups (KOG) 9620 (40.7%)
 BLASTx against AcariDB 18,245 (77.2%)
 Assigned with Gene Ontology (GO) termsc 11,360 (48.1%)
 Assigned with Enzyme Commission (EC) numbersc 3493 (14.8%)
 Assigned with KEGG orthology (KO) identifiersd 4869 (20.6%)
 Annotated in at least one database 18,311 (77.5%)
  1. aValue indicating either the number of transcripts, proteins or bases, the transcript length or percentage, as indicated in the table
  2. bNumber (and %) of transcripts annotated based on significant matches (E-value < E-05) against databases as detailed in the Methods section
  3. cGO terms and EC numbers assigned with Blast2GO
  4. dFrom the KEGG (Kyoto Encyclopedia of Genes and Genomes) Automatic Annotation Server (KAAS) using the I. scapularis genome