Sialotranscriptomics of Rhipicephalus zambeziensis reveals intricate expression profiles of secretory proteins and suggests tight temporal transcriptional regulation during blood-feeding

Table 1 Summary of the R. zambeziensis transcriptome assembly and annotation statistics

Transcriptome statistics	Value^a
Transcriptome assembly statistics
Total number of transcripts	23,631
Number of transcripts > 500 bp	19,903
Number of transcripts > 1 kb	13,330
Number of transcripts > 10 kb	80
Shortest transcript length (bp)	201
Longest transcript length (bp)	17,108
Mean length of transcripts (bp)	1793.5
Median length of transcripts (bp)	1193
Transcript N50 (bp)	2807
Total bases in assembly (Mb)	42.4
Ambiguous base calls (Ns)	0
GC content (%)	49
Number of non-redundant predicted proteins	13,584
Transcriptome annotation statistics^b
BLASTx against NR	12,756 (54.0%)
BLASTx against UniProtKB/TrEMBL	16,451 (69.6%)
BLASTx against UniProtKB/Swiss-Prot	10,572 (44.7%)
BLASTx against TSA-NR	16,711 (70.7%)
BLASTx against Ixodes scapularis predicted peptides	11,804 (50.0%)
BLASTx against EuKaryotic Orthologous Groups (KOG)	9620 (40.7%)
BLASTx against AcariDB	18,245 (77.2%)
Assigned with Gene Ontology (GO) terms^c	11,360 (48.1%)
Assigned with Enzyme Commission (EC) numbers^c	3493 (14.8%)
Assigned with KEGG orthology (KO) identifiers^d	4869 (20.6%)
Annotated in at least one database	18,311 (77.5%)

^aValue indicating either the number of transcripts, proteins or bases, the transcript length or percentage, as indicated in the table
^bNumber (and %) of transcripts annotated based on significant matches (E-value < E-05) against databases as detailed in the Methods section
^cGO terms and EC numbers assigned with Blast2GO
^dFrom the KEGG (Kyoto Encyclopedia of Genes and Genomes) Automatic Annotation Server (KAAS) using the I. scapularis genome

ISSN: 1756-3305