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Table 1 Summary of the R. zambeziensis transcriptome assembly and annotation statistics

From: Sialotranscriptomics of Rhipicephalus zambeziensis reveals intricate expression profiles of secretory proteins and suggests tight temporal transcriptional regulation during blood-feeding

Transcriptome statistics

Valuea

Transcriptome assembly statistics

 Total number of transcripts

23,631

 Number of transcripts > 500 bp

19,903

 Number of transcripts > 1 kb

13,330

 Number of transcripts > 10 kb

80

 Shortest transcript length (bp)

201

 Longest transcript length (bp)

17,108

 Mean length of transcripts (bp)

1793.5

 Median length of transcripts (bp)

1193

 Transcript N50 (bp)

2807

 Total bases in assembly (Mb)

42.4

 Ambiguous base calls (Ns)

0

 GC content (%)

49

 Number of non-redundant predicted proteins

13,584

Transcriptome annotation statisticsb

 BLASTx against NR

12,756 (54.0%)

 BLASTx against UniProtKB/TrEMBL

16,451 (69.6%)

 BLASTx against UniProtKB/Swiss-Prot

10,572 (44.7%)

 BLASTx against TSA-NR

16,711 (70.7%)

 BLASTx against Ixodes scapularis predicted peptides

11,804 (50.0%)

 BLASTx against EuKaryotic Orthologous Groups (KOG)

9620 (40.7%)

 BLASTx against AcariDB

18,245 (77.2%)

 Assigned with Gene Ontology (GO) termsc

11,360 (48.1%)

 Assigned with Enzyme Commission (EC) numbersc

3493 (14.8%)

 Assigned with KEGG orthology (KO) identifiersd

4869 (20.6%)

 Annotated in at least one database

18,311 (77.5%)

  1. aValue indicating either the number of transcripts, proteins or bases, the transcript length or percentage, as indicated in the table
  2. bNumber (and %) of transcripts annotated based on significant matches (E-value < E-05) against databases as detailed in the Methods section
  3. cGO terms and EC numbers assigned with Blast2GO
  4. dFrom the KEGG (Kyoto Encyclopedia of Genes and Genomes) Automatic Annotation Server (KAAS) using the I. scapularis genome