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Table 1 DTUs detected by PCR from blood samples, and from XD coupled with PCR (1XD, 2XD and 3XD), per individual Octodon degus analyzed

From: Within-host temporal fluctuations of Trypanosoma cruzi discrete typing units: the case of the wild reservoir rodent Octodon degus

       

1XD

  

2XD

  

3XD

 

Animal

Sex

Weight

qPCR (parasite equivalent/ml)

Inicial blood

Triatomine XD

40 days

80 days

120 days

40 days

80 days

120 days

40 days

80 days

120 days

2

F

112.4

3.54

+

Ms

II-V-VI

II-V

I-V

   

Ti

II-V

II-V

+

V-VI

3

M

172

4.4

I-II-V-VI

Ms

II-V

II-V

+

+

V

I

+

Ti

II-V

II-V

+

I-V

I

7

F

123.7

1.37

VI

Ms

+

+

+

+

V

V

V

Ti

V

V

V

V

8

F

184

3.54

II-V-VI

Ms

II-V-VI

II-V

+

      

Ti

II-V

10

M

191

14.728

II-V-VI

Ms

II-V

+

+

II

   

Ti

II-V

+

13

F

166

0.5114

II-V-VI

Ms

II-V

+

+

V-VI

V

Ti

II-V

V

V

V

22

F

132

21.94

II-V-VI

Ms

+

V

+

V-VI

Ti

+

VI

25

F

134

4.24

+

Ms

+

Ti

+

+

29

M

187

10.26

II-V

Ms

II-V

+

+

Ti

II-V

+

+

V-VI

31

F

149

3.96

V-VI

Ms

+

Ti

32

F

201

5.56

V

Ms

+

Ti

+

33

F

196

2.56

V-VI

Ms

+

+

Ti

+

34

F

184

6.78

II

Ms

+

+

+

V

+

Ti

+

+

+

V

V

I-V

40

M

133

11.54

V

Ms

+

+

+

+

+

I-II-V

Ti

+

+

+

+

+

I

I-II-V

41

M

120

5.68

II-V

Ms

+

+

+

Ti

+

+

+

V

42

M

171

9.5

II-V

Ms

+

I

Ti

+

+

+

+

+

43

M

107

10.72

II-V

Ms

+

+

V

+

V

+

+

I-II-V

I-V

Ti

+

+

+

+

II

I-II

50

M

125

812.2

I-II

Ms

+

+

V

V

+

I

II

Ti

+

+

+

+

I-II-V

51

M

129

7.24

II-V

Ms

I-V

I

V

+

V

Ti

I-V

V

+

+

V

52

M

141

28.74

II-V-VI

Ms

I-V

V

+

+

Ti

I-V

I-V

+

+

+

53

F

122

116.2

+

Ms

I-V

+

V

Ti

I-V

+

+

+

V

54

F

163

80.28

+

Ms

+

V

Ti

+

V

57

M

117

5.14

II-V

Ms

+

+

V

+

Ti

I-V

+

+

+

+

  1. Abbreviations: F female, M male, Ms Mepraia spinolai, Ti Triatoma infestans
  2. The “–” sign indicates a negative conventional PCR result. The “+” sign indicates a positive PCR result, but these samples did not hybridize with any of the probes used. The dark lockers indicate that the individual perished before performing the XD. Analyses 1, 2 and 3 correspond to post-XD feeding with Mus musculus at 40, 80 and 120 days, respectively