Detecting and characterizing mixed infections with genetic variants of Anaplasma phagocytophilum in roe deer (Capreolus capreolus) by developing an ankA cluster-specific nested PCR

Table 1 List and characteristics of primers designed in this study

Primer type	Primer name	Sequence 5′–3’	Cluster specificity^a	Melting temperature (°C)	Amplicon size (bp)
External primers	Ana-Ext-F2	TCTGMAAGCCATTATCAYAG	I, II, III, IV	58	1609
External primers	Ana-Ext-R1	TGCTTCACGAARCGCATA	I, II, III, IV	58	1609
Internal primers	Ana-Int-F1	CATCKAGCAKGTRTTGAAGG	I, II, III, IV	58	731–737
Internal primers	Ana-Int-R3	TGTARAGGRGCARMACC	I, II, III, IV	58	731–737
Sequencing primers	Ana-Int-F2	GCGAGTGTGCAGASKCACTA	I, II, III, IV	58	328
Sequencing primers	Ana-Int-R3	TGTARAGGRGCARMACC	I, II, III, IV	58	328
Internal cluster-specific primers	Ana-F-Cl2	GTGACAATTTTGRGACATTAC	II	60	1512
	Ana-R-Cl2	TCCTAGTCCGCGAGCTAAGT	II	60	1512
	Ana-F-Cl3	GTGAGAATTTTGAGTCATTG	III	60	1512
	Ana-R-Cl3	TCCGAACCCGRGCGCTCAT	III	60	1512
	Ana-F-Cl4b	GAGGTATCTAGGAAGTGCC	IV	61	1286
	Ana-R-Cl4	TCAAGTTCCGTGCGGTTAGG	IV	61	1286

ISSN: 1756-3305