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Fig. 4 | Parasites & Vectors

Fig. 4

From: Plasmodium berghei Cap93, a novel oocyst capsule-associated protein, plays a role in sporozoite development

Fig. 4

Characterization of PbCap93 KO parasites. a Schematic of targeted disruption of the PbCap93 gene. The targeting vector (middle) containing a selectable marker gene was integrated into the PbCap93 gene locus (top) by double crossover. This recombination event resulted in the disruption of the PbCap93 gene and conferred pyrimethamine resistance (bottom). b PCR analysis of wild-type (WT) and PbCap93-KO parasites. Integration-specific PCR primer sets were used: P93F and P93R, P93F and DHFR-R1, and P93R and DHFR-F1. c Genomic Southern blot hybridization of WT and PbCap93-KO parasites. Parasite genomic DNA was digested with SpeI/HpaI and hybridized with the probe indicated by a solid bar (blue and red) in a. By integration of the targeting construct, the size of detected fragments increased from 2.5 to 3.5 kbp (blue) and 3.5 kbp (red). d Semi-quantification of PbCap93 mRNA abundance. Semi-quantitative RT-PCR was performed using cDNA prepared from midguts of mosquitoes infected with either WT or PbCap93-KO parasites at 10 days post-infection

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