Natural antibody responses to Plasmodium falciparum MSP3 and GLURP(R0) antigens are associated with low parasite densities in malaria patients living in the Central Region of Ghana

Table 1 msp1 and msp2 genotyping primers

Marker	Primer name	Primer sequence (5′-3′)	Annealing temperature (°C)
msp1
Primary	M1- OF	CTAGAAGCTTTAGAAGATGCAGTATTG	54
Primary	M1- OR	CTTAAATAGTATTCTAATTCAAGTGGATCA	54
K1	M1- KF	AAATGAAGAAGAAATTACTACAAAAGGTGC	59
K1	M1- KR	GCTTGCATCAGCTGGAGGGCTTGCACCAGA	59
MAD 20	M1- MF	AAATGAAGGAACAAGTGGAACAGCTGTTAC	59
MAD 20	M1- MR	ATCTGAAGGATTTGTACGTCTTGAATTACC	59
RO33	M1- RF	TAAAGGATGGAGCAAATACTCAAGTTGTTG	59
RO33	MI- RR	CAAGTAATTTTGAACTCATGTTTTAAATCAGCGTA	59
msp2
Primary	M2- OF	ATGAAGGTAATTAAAACATTGTCTATTATA	54
Primary	M2- OR	CTTTGTTACCATCGGTACATTCTT	54
3D7/FC27- F	S1fw	GCTTATAATATGAGTATAAGGAGAA	50
FC27- R	M5rev	GCATTGCCAGAACTTGAA
3D7 - R	N5rev	CTGAAGAGGTACTGGTAGA

ISSN: 1756-3305