Fig. 3From: Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectorsRepresentative gel for molecular detection of T. cruzi DNA in field triatomine samples by multiplex conventional PCR. Lane M: 100Â bp molecular marker; Lanes 1, 2: PCR negative controls (PCR reagents without DNA and ultra-pure water, respectively); Lane 3: DNA extraction negative control (extraction reagents in ultrapure water); Lanes 4, 12: field triatomine samples; Lane 13: multiplex PCR positive control (R. prolixus midgut spiked with ~102 CL Brener epimastigotes); Lane 14: T. cruzi positive control (DNA extracted from 102 CL Brener epimastigotes); Lane 15: T. rangeli positive control (DNA extracted from 102 Macias strain epimastigotes)Back to article page