Fig. 5From: Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectorsReportable range for T. cruzi and triatomine intestine unit quantification by real-time qPCR. Multiplex TaqMan qPCR assays were carried out with serially diluted DNA extracted from reconstituted triatomine intestine samples containing T. cruzi epimastigotes, ranging from 105 to 0.5Â T. cruzi equivalents (a) and 5 to 0.002 triatomine intestine unit equivalents (b). The slope, R2 and amplification efficiency (Eff) are indicated in the chartBack to article page