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Table 3 Representation of Cq mean values for detection of the 12S rDNA gene by real-time PCR in distinct triatomine species and absence of amplification signal in animal blood samples

From: Development of conventional and real-time multiplex PCR-based assays for estimation of natural infection rates and Trypanosoma cruzi load in triatomine vectors

Triatomine detection (1 intestine, 8–10 ng DNA)

Possible blood-meal source detection (blood, 20 ng DNA)

Triatomine species

Cq (Mean ± SD)

Origin

Cq (Mean ± SD)

Rhodnius prolixus

25.54 ± 0.30

Human

nd

Rhodnius neglectus

26.55 ± 0.54

Bat

nd

Triatoma vitticeps

26.86 ± 1.37

Cat

nd

Triatoma sordida

24.54 ± 0.39

Dog

nd

  

Opossum

nd

  

Rat

nd

  1. Abbreviations: C q threshold cycle, SD standard deviation, nd not detected