Table 4 SAT-DNA qPCR positivity to estimate the limit of detection (LOD) for T. cruzi DNA in triatomine samples
Parasite load (T. cruzi equivalents) | Assay # | Positive samples (%) | Triatomine internal reference (Cq ± SD) |
|---|---|---|---|
20 | 1 | 12 (100) | 21.19 ± 0.39 |
2 | 12 (100) | ||
3 | 12 (100) | ||
4 | 1 | 12 (100) | 22.09 ± 1.85 |
2 | 12 (100) | ||
3 | 12 (100) | ||
0.8 | 1 | 12 (100) | 21.48 ± 0.86 |
2 | 12 (100) | ||
3 | 12 (100) | ||
0.16 | 1 | 10 (83.3) | 22.56 ± 1.40 |
2 | 8 (66.7) | ||
3 | 10 (83.3) | ||
0.032 | 1 | 11 (91.7) | 22.65 ± 1.12 |
2 | 10 (83.3) | ||
3 | 5 (41.7) | ||
0.0064 | 1 | 7 (58.3) | 23.06 ± 1.51 |
2 | 6 (50.0) | ||
3 | 6 (50.0) | ||
0.00128 | 1 | 1 (8.3) | 22.39 ± 0.76 |
2 | 1 (8.3) | ||
3 | – |