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Table 3 Genetic diversity indices and neutrality tests (Fu’s Fs and Tajima’s D) of the ITS2 gene in 17 Hyrcanus Group members

From: Molecular phylogeny of Anopheles hyrcanus group members based on ITS2 rDNA

Species n S Pi h Hd Fu’s Fs Tajima’s D
An. argyropus 18 4 0.00289 4 0.477 0.584 0.49002
An. nigerrimus 26 2 0.00195 2 0.492 3.173 1.88188
An. nitidus 43 3 0.00222 3 0.638 2.118 1.12137
An. pursati 22 1
An. pseudopictus 30 1
An. hyrcanus 48 9 0.00140 5 0.268 -1.517 -1.98082*
An. hyrcanus_spIR 7 5 0.00497 3 0.524 1.508 0.36328
An. pullus 23 4 0.00304 6 0.775 -1.211 0.60421
An. lesteri 18 42 0.02042 6 0.490 4.914* -1.33520
An. paraliae 25 1
An. crawfordi 53 12 0.01115 4 0.491 9.501** 2.50567*
An. liangshanensis 4 1 0.00114 2 0.500 0.172 -0.61237
An. sineroides 4 1 0.00120 2 0.500 0.172 -0.61237
An. sinensis 41 5 0.00340 4 0.450 1.599 0.43762
An. belenrae 5 1
An. kleini 9 1
An. peditaeniatus 83 13 0.00094 9 0.185 -8.629** -2.36082**
  1. The significance of Fu’s Fs and Tajima’s D values is indicated by asterisks (*P < 0.05, **P < 0.01)
  2. Species represented by < 3 specimens were excluded from the analyses
  3. Abbreviations: n number of sequences, S number of polymorphic sites, pi nucleotide diversity, h number of haplotypes, Hd haplotype diversity