Fig. 1

Binding of rMNh and rMCh to goat PBMC. The immunofluorescence assay was carried out by incubation of cells with rat anti-MNh/MCh IgG or negative rat IgG (Control). DAPI (blue) and Cy3-conjugated secondary antibodies (red) were utilized for double staining. Merge, overlap of Cy3, DAPI and DIC channels. a PBMC pretreated with rMNh were incubated with negative rat IgG (Control). b PBMC pretreated with rMNh were incubated with rat anti-MNh IgG. c PBMC pretreated with rMCh were incubated with negative rat IgG (Control). d PBMC pretreated with rMCh were incubated with rat anti-MCh IgG. e PBMC pretreated with empty recombinant pET-32a protein were incubated with negative rat IgG (Control). f PBMC pretreated with empty recombinant pET-32a protein were incubated with rat anti-pET-32a protein IgG