Fig. 3

Immunohistochemical localization of Fas1 and Fas2 proteins in T. solium metacestode (TsM). a, b TsM sections (4 μm thick) were incubated with anti-rTsMFas1 or anti-rTsMFas2 antibody (1:200 dilution) and further incubated with FITC-conjugated anti-mouse IgG antibody (1:500 dilution). Areas marked by a, b and c are also shown in highlight views. White arrowheads show positive reactions to calcareous corpuscles (CC). Abbreviations: BW, bladder wall; CA, spiral canal; CF, cyst fluid; SC, scolex. Scale-bars: 200 μm. c TsM sections were probed with control IgG isolated from preimmune mouse serum (1:200 dilution) and subsequently with FITC-conjugated anti-mouse IgG antibody (1:500 dilution). Markings are the same as described in a. d In vitro binding of rTsMFas1 or rTsMFas2 with CC. rTsMFas 1 or 2 protein (each 10 μg) was incubated with CC (10 μl). The binding complex was precipitated, resuspended in 2× reducing sample buffer and separated by 8% SDS-PAGE. Proteins were transblotted to nitrocellulose membranes and probed with respective antibodies (1:2000 dilution). Immune signals were detected by ECL after 2 min exposure. Lane SN: scolex/neck proteins (10 μg); Lane CC + rTsMFas1 or 2: purified CC (each 10 μl) was incubated with rTsMFas1 or 2 (10 μg each); Lane CC: calcareous corpuscles only (10 μl). Abbreviation: M _r, molecular weight in kDa