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Fig. 5 | Parasites & Vectors

Fig. 5

From: Characterization of a secreted cystatin of the parasitic nematode Haemonchus contortus and its immune-modulatory effect on goat monocytes

Fig. 5

Uptake of rHCcyst-3 by goat monocytes. Goat monocytes were left untreated or incubated with rHCcyst-3 (40 μg/ml) for 30 min at 37 °C. All cells were fixed and incubated with rat anti-rHCcyst-3 antibody followed by Cy3 labeled goat anti-rat IgG (red). The nuclei and membranes of the corresponding cells were visualized by DAPI (blue) and DIO (green) staining, respectively. The internalization of rHCcyst-3 by goat monocytes was visualized with a confocal laser scanning microscopy. Merge, overlap of red and green channels. The data are representative of three independent experiments. Scale-bars: 2 μm

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