Fig. 8From: Characterization of a secreted cystatin of the parasitic nematode Haemonchus contortus and its immune-modulatory effect on goat monocytesrHCcyst-3 enhance NO production on LPS treated goat monocytes. Monocytes were stimulated with LPS (100Â ng/ml) for 48Â h in the presence or absence of rHCcyst-3 and his-tagged protein. The cells treated with rHCcyst-3 alone as another control. NO was measured in the cell supernatants as nitrite using a NO assay kit. The data are representative of three independent experiments (*PÂ <Â 0.05, **PÂ <Â 0.01, ***PÂ <Â 0.001)Back to article page