Fig. 9From: Characterization of a secreted cystatin of the parasitic nematode Haemonchus contortus and its immune-modulatory effect on goat monocytesrHCcyst-3 decrease phagocytic capacity of goat monocytes. Monocytes were collected after treated with rHCcyst-3 or his-tagged protein for 48 h and incubated with FITC-dextran (1 mg/ml in RPMI1640) for 1 h at 37 °C. The FITC-dextran internalization of cells was analyzed by flow cytometry and calculated as mean fluorescence intensity (MFI). The data are representative of three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001)Back to article page