Table 2 Primer combinations evaluated during the assay development
Primer combination | Amplification success | Product size (bp) |
|---|---|---|
F + R | Successful amplification; annealing at 55 °C | 76 |
F + R3 | Successful amplification; annealing at 55 °C | 95 |
F + R4 | Successful amplification; annealing at 55 °C | 257 |
F2 + R | Successful amplification; annealing at 52 °C | 100 |
F2 + R3 | Successful amplification; annealing at 52 °C | 119 |
F2 + R4 | Successful amplification; annealing at 52 °C | 281 |
F3 + R | Failed amplification | 40 |
F3 + R3 | Failed amplification | 59 |
F3 + R4 | Successful amplification; annealing at 52 °C | 221 |