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Table 2 Primer combinations evaluated during the assay development

From: SYBR green-based one step quantitative real-time polymerase chain reaction assay for the detection of Zika virus in field-caught mosquitoes

Primer combination Amplification success Product size (bp)
F + R Successful amplification; annealing at 55 °C 76
F + R3 Successful amplification; annealing at 55 °C 95
F + R4 Successful amplification; annealing at 55 °C 257
F2 + R Successful amplification; annealing at 52 °C 100
F2 + R3 Successful amplification; annealing at 52 °C 119
F2 + R4 Successful amplification; annealing at 52 °C 281
F3 + R Failed amplification 40
F3 + R3 Failed amplification 59
F3 + R4 Successful amplification; annealing at 52 °C 221