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Table 3 Comparison of the sensitivity of ZIKV detection between a probe-based rRT-PCR assay and the SYBR Green-based assay utilising the primer combination F2 + R3

From: SYBR green-based one step quantitative real-time polymerase chain reaction assay for the detection of Zika virus in field-caught mosquitoes

ZIKV strainb Mosquito species Detection threshold of virus titre (PFU/ml)
Probe-based rRT-PCRa SYBR Green-based rRT-PCR
ATCC® VR-84 Aedes aegypti 100 10
Aedes albopictus 10 1
Culex quinquefasciatus 10 100
PLCal_ZV Aedes aegypti 1 1
Aedes albopictus 0.1 0.1
Culex quinquefasciatus 10 10
  1. aThe assay protocol has previously been described by Lanciotti et al. [9]
  2. bZIKV strain ATCC® VR-84 is the prototype strain isolated in Uganda in 1947 (NCBI accession No. NC012532.1). The strain belonging to African genotype. PLCal_ZV strain was isolated in a Canadian traveller from Thailand in 2013 (NCBI accession No. KF993678). PLCal_ZV belongs to the Asian genotype