SYBR green-based one step quantitative real-time polymerase chain reaction assay for the detection of Zika virus in field-caught mosquitoes

Table 3 Comparison of the sensitivity of ZIKV detection between a probe-based rRT-PCR assay and the SYBR Green-based assay utilising the primer combination F2 + R3

ZIKV strain^b	Mosquito species	Detection threshold of virus titre (PFU/ml)
ZIKV strain^b	Mosquito species	Probe-based rRT-PCR^a	SYBR Green-based rRT-PCR
ATCC® VR-84	Aedes aegypti	100	10
	Aedes albopictus	10	1
	Culex quinquefasciatus	10	100
PLCal_ZV	Aedes aegypti	1	1
	Aedes albopictus	0.1	0.1
	Culex quinquefasciatus	10	10

^aThe assay protocol has previously been described by Lanciotti et al. [9]
^bZIKV strain ATCC® VR-84 is the prototype strain isolated in Uganda in 1947 (NCBI accession No. NC012532.1). The strain belonging to African genotype. PLCal_ZV strain was isolated in a Canadian traveller from Thailand in 2013 (NCBI accession No. KF993678). PLCal_ZV belongs to the Asian genotype

ISSN: 1756-3305