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Table 4 Evaluation of SYBR Green-based rRT-PCR assay performance in ZIKV infected field caught mosquitoes confirmed by a probe-based rRT-PCR assay

From: SYBR green-based one step quantitative real-time polymerase chain reaction assay for the detection of Zika virus in field-caught mosquitoes

Sample IDb Probe-based rRT-PCRa SYBR Green-based rRT-PCR
Cq value Result Cq value (Tm) Result
HT55 21.06 Positive 17.57 (80.38) Positive
HT66 19.54 Positive 15.23 (80.24) Positive
HT67 28.77 Positive 26.06 (80.16) Positive
HT68 29.19 Positive 26.98 (80.22) Positive
HT74 17.09 Positive 13.74 (80.42) Positive
HT81 19.66 Positive 16.33 (80.1) Positive
HT83 33.23 Positive 35 (80.04) Positive
Ae. albopictus Not available Negative Not available Negative
Ae. albopictus Not available Negative Not available Negative
Culex spp. Not available Negative Not available Negative
Culex spp. Not available Negative Not available Negative
Ae. aegypti Not available Negative Not available Negative
Ae. aegypti Not available Negative Not available Negative
Ae. aegypti Not available Negative Not available Negative
ZIKV positive control (ATCC® VR-84) 16.77 Positive 13.84 (79.88) Positive
Negative control Not available Negative Not available Negative
  1. Abbreviations: C q quantification cycle, T m melting temperature
  2. aThe assay protocol has previously been described by Lanciotti et al. [9]
  3. bSample IDs from HT55 to HT83 are Ae. aegypti specimens collected from a zika fever cluster during the first outbreak of ZIKV in Singapore in August 2016 [6]. They were confirmed to be ZIKV-positive by the probe-based rRT-PCR assay. ZIKV negative mosquito specimens collected from the field, including the same cluster, are included for the comparison of specificity