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Table 2 Detection of S. lupi eggs in fecal samples from dogs by five different assays

From: Detection and quantification of Spirocerca lupi by HRM qPCR in fecal samples from dogs with spirocercosis

Fecal sample code number HRM qPCR for ITS1 (estimated epg) HRM qPCR for 18S (estimated epg) HRM qPCR for cytb (estimated epg) Semi-nested PCR for cox1 Fecal flotation (estimated epg)
A-01F + (13.1 ± 9.0) + (2.2 ± 0.1) + (120)
C-01F
D-01F + (106.8 ± 45.5) + (28.5 ± 8.2) + (5.7 ± 1.8) + + (10)
E-01F + (0.7 ± 0.1) + (0.03 ± 0.001) + (12)
F-01F
G-01F
I-01F + (7.8 ± 1.0) + (0.3 ± 0.04)
J-01F + (0.7 ± 0.1)
K-01F + (0.5 ± 0.1)
L-01F
M-01F + (0.7 ± 0.1)
N-01F + (2.3 ± 0.5) + (0.3 ± 0.1) + (1)
O-01F + (41.4 ± 9.8) + (17.0 ± 3.1) + (0.1 ± 0.06) + (160)
P-01F
Q-01F
S-01F
T-01F + (8.6 ± 2.2) + (0.97 ± 1.1)
U-01F + (12.3 ± 1.5) + (4.4 ± 0.1)
Total 11 8 2 1 5