O-5S quantitative real-time PCR: a new diagnostic tool for laboratory confirmation of human onchocerciasis

Table 1 Sequences and nucleotide positions of primers and the hydrolysis probe, including the corresponding amplicon sizes of the O-5S qPCR

Test	Primer/probe^a	Sequence (5'-3')^b	Nucleotide position^c	Amplicon size (bp)
O-5S qPCR	Ov5S-F	GAGGTAATTGAATGTTTCTGCCC	74–96	84
	Ov5S-R	TGTTGTCCCGCTCATGC	158–142
	Ov5S-P	FAM-AGTTTCGACTGCTGTGGCTTGAAGCG-BHQ1	99–124

^aF, forward primer; R, reverse primer; P, hydrolysis probe (TibMolBiol, Berlin, Germany)
^bHydrolysis probe with Fluorescein (FAM) and BlackHole Dark Quencher (BHQ)
^cNucleotide positions are provided for the intergenic spacer region of the 5S ribosomal RNA gene of Onchocerca volvulus (GenBank: U31643.1)