Fig. 2From: Stimulation of IL-1β and IL-6 through NF-κB and sonic hedgehog-dependent pathways in mouse astrocytes by excretory/secretory products of fifth-stage larval Angiostrongylus cantonensis ESP stimulate the gene expression of Shh signaling pathway. Astrocytes were treated with 500 μg/ml ESP for the indicated time points. The mRNA levels of Shh, Ptch, and Gli-1 were determined in astrocytes by Quantitative real-time PCR. Statistical significance was determined by Student’s t-test: *P < 0.05, **P < 0.01, ***P < 0.001 (n = 3)Back to article page