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Fig. 4 | Parasites & Vectors

Fig. 4

From: Stimulation of IL-1β and IL-6 through NF-κB and sonic hedgehog-dependent pathways in mouse astrocytes by excretory/secretory products of fifth-stage larval Angiostrongylus cantonensis

Fig. 4

ESP induce the expressions of IL-1β and IL-6 via the Shh pathway. Western blot analysis of IL-1β (a) and IL-6 (b) in astrocytes co-cultured with ESP alone or pretreated with a recombinant Sonic hedgehog peptide from mouse (Shh) (3 μg), cyclopamine (20 μM) or SAG for 2 h and then with 500 μg/ml ESP for 4 h. β-actin is shown as a control. Data are expressed as the mean ± SD from three independent experiments (**P < 0.01). (c, d) Changes in the concentrations of IL-1β and IL-6 protein in the culture medium of astrocytes detected by the ELISA. Data are expressed as the mean ± SD from three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001)

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