Table 1 Real-time quantitative PCR primer sequences
Gene | Direction | Sequence (5′-3′) |
|---|---|---|
GAPDH | Forward | AACTTTGGCATTGTGGAAGG |
Reverse | GGATGCAGGGATGATGTTCT | |
IRS-1 | Forward | GGTCACGAAGAGCTGGTGCACT |
Reverse | GAGGCGCATCATGGAACACGG | |
IRS-2 | Forward | GCTCCCTGTTCCTGCAGCGG |
Reverse | CAAAGGTGCCAGCCCCTGGG | |
INSR | Forward | GATGTGCACCCCATGTCTG |
Reverse | CTGAATAGCTGAGACCACAG | |
G6PC | Forward | TTACCAAGACTCCCAGGACTG |
Reverse | GAGCTGTTGCTGTAGTAGTCG | |
GLUT4 | Forward | TGTATGTGGGAGAAATCGC |
Reverse | GACAGAAGGGCAGCAGAAT | |
TNF-α | Forward | CATCTTCTCAAAATTCGAGTGACAA |
Reverse | TGGGAGTAGACAAGGTACAACCC | |
IL-6 | Forward | GAGGATACCACTCCCAACAGACC |
Reverse | AAGTGCATCATCGTTGTTCATACA | |
IL-1β | Forward | GCACACCCACCCTGCA |
Reverse | ACCGCTTTTCCATCTTCTTCTT | |
TGF-β1 | Forward | ATGCTAAAGAGGTCAAAAGC |
Reverse | CCAAGGTAACGCCAGGAATT | |
IL-10 | Forward | ACTTTAAGGGTTACTTGGGTTGC |
Reverse | ATTTTACAAGGGGAGAAATCG | |
IL-13 | Forward | GCCAGCCCACAGTTCTAC |
Reverse | GAGATGTTGGTCAGGGAAT | |
IL-22 | Forward | GTCAACCGCACCTTTATGCT |
Reverse | CATGTAGGGCTGGAACCTGT | |
IL-33 | Forward | GATGGGAAGAAGGTGATGGTG |
Reverse | TTGTGAAGGACGAAGAAGGC |