Fig. 2From: Moroccan strains of Leishmania major and Leishmania tropica differentially impact on nitric oxide production by macrophagesEffect of L. major and L. tropica promastigotes on NO production by macrophages. Macrophages were infected with 10 promastigotes of L. tropica or L. major /cell for 4 h. Cells were kept either without stimulus or stimulated with IFN-γ (10 ng/ml) and LPS (50 ng/ml) in presence or absence of L-NMMA (1 mg/ml). The supernatants were collected and nitrite concentrations were evaluated by Griess reaction at 24 h post-infection (a) and 48 h post-infection (b). Results are expressed as mean ± SEM from six independent experiments, each performed in duplicate. Data were analyzed using one way ANOVA test with Tukey’s post-hoc test for multiple comparisons. *P < 0.05, **P < 0.01. Abbreviation: ns, not significantBack to article page