Fig. 4From: Differential susceptibility of bovine caruncular and trophoblast cell lines to infection with high and low virulence isolates of Neospora caninum Proliferation kinetics over time and tachyzoite yield at 58 hpi. Graphs (a and b) represent the average number of tachyzoites for each time-point for all individual experiments with an R2 > 0.95, except for BCEC-1 cells infected by Nc-Spain1H, which showed a non-exponential growth pattern. Error bars indicate the SD. Representative images (c) show the proliferation kinetics over time of Nc-Spain7 and Nc-Spain1H isolates in F3 and BCEC-1 cultures. The bar graph (d) represents the tachyzoite yield at 58 hpi for Nc-Spain7 and Nc-Spain1H in F3 and BCEC-1 cells. Each column and error bar represents the mean and the SD of 4 replicates from 2 independent assays. The TY58h was fifteen times higher in F3 cells than in BCEC-1 cells infected with Nc-Spain7, and ten times higher in F3 cells infected with Nc-Spain1H. Statistical differences were found in the TY58h between isolates in F3 cells, with the TY58h of Nc-Spain7 significantly higher than for Nc-Spain1H (P < 0.0001). * represents significant differences between isolates. Scale-bars: c, 10 μmBack to article page