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Fig. 4 | Parasites & Vectors

Fig. 4

From: Differential susceptibility of bovine caruncular and trophoblast cell lines to infection with high and low virulence isolates of Neospora caninum

Fig. 4

Proliferation kinetics over time and tachyzoite yield at 58 hpi. Graphs (a and b) represent the average number of tachyzoites for each time-point for all individual experiments with an R2 > 0.95, except for BCEC-1 cells infected by Nc-Spain1H, which showed a non-exponential growth pattern. Error bars indicate the SD. Representative images (c) show the proliferation kinetics over time of Nc-Spain7 and Nc-Spain1H isolates in F3 and BCEC-1 cultures. The bar graph (d) represents the tachyzoite yield at 58 hpi for Nc-Spain7 and Nc-Spain1H in F3 and BCEC-1 cells. Each column and error bar represents the mean and the SD of 4 replicates from 2 independent assays. The TY58h was fifteen times higher in F3 cells than in BCEC-1 cells infected with Nc-Spain7, and ten times higher in F3 cells infected with Nc-Spain1H. Statistical differences were found in the TY58h between isolates in F3 cells, with the TY58h of Nc-Spain7 significantly higher than for Nc-Spain1H (P < 0.0001). * represents significant differences between isolates. Scale-bars: c, 10 μm

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