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Table 1 Summary of virulence traits as a function of cell type and N. caninum isolate

From: Differential susceptibility of bovine caruncular and trophoblast cell lines to infection with high and low virulence isolates of Neospora caninum

   pInvRa cInfRb % Multi-infectionc Invasion efficiencyd TY58H e Td f
Cell type comparisons
(F3 vs BCEC-1)
Nc-Spain7 + ++++ + - - - - + - -
Nc-Spain1H NSh ++++ + - - - - + NCg
Isolate comparisons
(Nc-Spain7 vs Nc-Spain1H)
F3 ++ + ++++ ++++ ++ NS
BCEC-1 NS + NS NS NS NCg
  1. apInvR (parasite invasion rate): number of tachyzoites invading the host cell at different time points post-infection
  2. bcInfR (cell infection rate): percentage of cells infected using different parasite doses
  3. c% Multi-infection: percentage of cells containing more than one vacuole
  4. dInvasion efficiency: results from adhesion-invasion assay, percentage of intracellular tachyzoites relative to the total number of tachyzoites at 4 hpi
  5. eTY58H (tachyzoite yield at 58 hpi): average number of tachyzoites quantified by qPCR at 58 hpi
  6. fTd (Doubling time): period of time required for a tachyzoite to duplicate during the exponential multiplication period, excluding lag and egress phases
  7. g NC data not comparable. The Td value for Nc-Spain1H isolate in BCEC-1 cells could not be calculated due to the lack of exponential growth of Nc-Spain1H in BCEC-1
  8. h NS no significant differences
  9. +/++/++++ indicate higher rates of each parameter assayed with a significance of P < 0.05, P < 0.01, P < 0.0001, respectively
  10. -/- -/- - - - indicate lower rates of each parameter assayed with a significance of P < 0.05, P < 0.01, P < 0.0001, respectively