| |
pInvRa
|
cInfRb
|
% Multi-infectionc
|
Invasion efficiencyd
|
TY58H
e
|
Td
f
|
---|
Cell type comparisons
(F3 vs BCEC-1)
|
Nc-Spain7
|
+
|
++++
|
+
|
- - - -
|
+
|
- -
|
Nc-Spain1H
|
NSh
|
++++
|
+
|
- - - -
|
+
|
NCg
|
Isolate comparisons
(Nc-Spain7 vs Nc-Spain1H)
|
F3
|
++
|
+
|
++++
|
++++
|
++
|
NS
|
BCEC-1
|
NS
|
+
|
NS
|
NS
|
NS
|
NCg
|
-
apInvR (parasite invasion rate): number of tachyzoites invading the host cell at different time points post-infection
-
bcInfR (cell infection rate): percentage of cells infected using different parasite doses
-
c% Multi-infection: percentage of cells containing more than one vacuole
-
dInvasion efficiency: results from adhesion-invasion assay, percentage of intracellular tachyzoites relative to the total number of tachyzoites at 4 hpi
-
eTY58H (tachyzoite yield at 58 hpi): average number of tachyzoites quantified by qPCR at 58 hpi
-
fTd (Doubling time): period of time required for a tachyzoite to duplicate during the exponential multiplication period, excluding lag and egress phases
-
g
NC data not comparable. The Td value for Nc-Spain1H isolate in BCEC-1 cells could not be calculated due to the lack of exponential growth of Nc-Spain1H in BCEC-1
-
h
NS no significant differences
- +/++/++++ indicate higher rates of each parameter assayed with a significance of P < 0.05, P < 0.01, P < 0.0001, respectively
- -/- -/- - - - indicate lower rates of each parameter assayed with a significance of P < 0.05, P < 0.01, P < 0.0001, respectively