Skip to main content

Table 1 Summary of virulence traits as a function of cell type and N. caninum isolate

From: Differential susceptibility of bovine caruncular and trophoblast cell lines to infection with high and low virulence isolates of Neospora caninum

  

pInvRa

cInfRb

% Multi-infectionc

Invasion efficiencyd

TY58H e

Td f

Cell type comparisons

(F3 vs BCEC-1)

Nc-Spain7

+

++++

+

- - - -

+

- -

Nc-Spain1H

NSh

++++

+

- - - -

+

NCg

Isolate comparisons

(Nc-Spain7 vs Nc-Spain1H)

F3

++

+

++++

++++

++

NS

BCEC-1

NS

+

NS

NS

NS

NCg

  1. apInvR (parasite invasion rate): number of tachyzoites invading the host cell at different time points post-infection
  2. bcInfR (cell infection rate): percentage of cells infected using different parasite doses
  3. c% Multi-infection: percentage of cells containing more than one vacuole
  4. dInvasion efficiency: results from adhesion-invasion assay, percentage of intracellular tachyzoites relative to the total number of tachyzoites at 4 hpi
  5. eTY58H (tachyzoite yield at 58 hpi): average number of tachyzoites quantified by qPCR at 58 hpi
  6. fTd (Doubling time): period of time required for a tachyzoite to duplicate during the exponential multiplication period, excluding lag and egress phases
  7. g NC data not comparable. The Td value for Nc-Spain1H isolate in BCEC-1 cells could not be calculated due to the lack of exponential growth of Nc-Spain1H in BCEC-1
  8. h NS no significant differences
  9. +/++/++++ indicate higher rates of each parameter assayed with a significance of P < 0.05, P < 0.01, P < 0.0001, respectively
  10. -/- -/- - - - indicate lower rates of each parameter assayed with a significance of P < 0.05, P < 0.01, P < 0.0001, respectively