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Table 2 Comparison of genotyping results for V1016G and F1534C mutations from multiplex PCR and DNA sequencing

From: A multiplex PCR for detection of knockdown resistance mutations, V1016G and F1534C, in pyrethroid-resistant Aedes aegypti

Strain Year of collection Multiplex PCR genotyping/DNA sequencing (no. of samples)
VV/FF VV/FC VV/CC VG/FF VG/FC VG/CC GG/FF GG/FC GG/CC Total
Laboratory strains
 PMD   10/10 0/0 0/0 0/0 0/0 0/0 0/0 0/0 0/0 10/10
 PMD-R   0/0 0/0 10/10 0/0 0/0 0/0 0/0 0/0 0/0 10/10
 UPK-R   0/0 0/0 0/0 0/0 0/0 0/0 10/10 0/0 0/0 10/10
 (PMDxUPK-R) F1 hybrid   0/0 0/0 0/0 10/10 0/0 0/0 0/0 0/0 0/0 10/10
 (PMD-RxUPK-R) F1 hybrid   0/0 0/0 0/0 0/0 10/10 0/0 0/0 0/0 0/0 10/10
 YG   0/0 0/0 1/1 0/0 1/1 0/0 5/5 3/3 0/0 10/10
 MS   0/0 0/0 0/0 0/0 2/2 2/2 0/0 5/5 1/1 10/10
Field-collected strains
Thailand
 Chiang Mai city, Chiang Mai 2016 0/0 0/0 15/15 0/0 20/20 0/0 12/12 0/0 0/0 47/47
 Mae Taeng district, Chiang Mai 2016 0/0 0/0 12/12 0/0 5/5 0/0 1/1 0/0 0/0 18/18
 Mae Sariang district, Mae Hong Sona 2010 0/0 2/2 6/6 0/0 6/6 0/0 2/2 0/0 0/0 16/16
Total   10/10 2/2 44/44 10/10 44/44 2/2 30/30 8/8 1/1 151/151
  1. aMosquito DNA samples were obtained from Yanola et al. [10]