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Table 2 Comparison of genotyping results for V1016G and F1534C mutations from multiplex PCR and DNA sequencing

From: A multiplex PCR for detection of knockdown resistance mutations, V1016G and F1534C, in pyrethroid-resistant Aedes aegypti

Strain

Year of collection

Multiplex PCR genotyping/DNA sequencing (no. of samples)

VV/FF

VV/FC

VV/CC

VG/FF

VG/FC

VG/CC

GG/FF

GG/FC

GG/CC

Total

Laboratory strains

 PMD

 

10/10

0/0

0/0

0/0

0/0

0/0

0/0

0/0

0/0

10/10

 PMD-R

 

0/0

0/0

10/10

0/0

0/0

0/0

0/0

0/0

0/0

10/10

 UPK-R

 

0/0

0/0

0/0

0/0

0/0

0/0

10/10

0/0

0/0

10/10

 (PMDxUPK-R) F1 hybrid

 

0/0

0/0

0/0

10/10

0/0

0/0

0/0

0/0

0/0

10/10

 (PMD-RxUPK-R) F1 hybrid

 

0/0

0/0

0/0

0/0

10/10

0/0

0/0

0/0

0/0

10/10

 YG

 

0/0

0/0

1/1

0/0

1/1

0/0

5/5

3/3

0/0

10/10

 MS

 

0/0

0/0

0/0

0/0

2/2

2/2

0/0

5/5

1/1

10/10

Field-collected strains

Thailand

 Chiang Mai city, Chiang Mai

2016

0/0

0/0

15/15

0/0

20/20

0/0

12/12

0/0

0/0

47/47

 Mae Taeng district, Chiang Mai

2016

0/0

0/0

12/12

0/0

5/5

0/0

1/1

0/0

0/0

18/18

 Mae Sariang district, Mae Hong Sona

2010

0/0

2/2

6/6

0/0

6/6

0/0

2/2

0/0

0/0

16/16

Total

 

10/10

2/2

44/44

10/10

44/44

2/2

30/30

8/8

1/1

151/151

  1. aMosquito DNA samples were obtained from Yanola et al. [10]