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Table 3 Comparison of genotyping results for V1016G and F1534C mutations from multiplex PCR and AS-PCR

From: A multiplex PCR for detection of knockdown resistance mutations, V1016G and F1534C, in pyrethroid-resistant Aedes aegypti

Strain Year of collection Total Multiplex PCR genotyping/AS-PCR genotyping (No. of samples)
1534 1016
F/F F/C C/C V/V V/G G/G
Thailand
 Mae Sariang district, Mae Hong Sona 2010 15 4/4 5/4 6/7c 9/9 2/2 4/4
 Ranot district, Song Khlaa 2008 10 0/0 3/3 7/7 10/10 0/0 0/0
 Ubon Ratchathani city, Ubon Ratchathania 2008 9 0/0 1/1 8/8 8/8 1/1 0/0
Myanmar
 Yangon citya 2008 10 4/4 6/6 0/0 10/10 0/0 0/0
 Yangon city 2016 32 15/15 16/16 1/1 0/0 0/0 32/32
Cambodia
 Battambang citya 2008 10 0/0 0/0 10/10 10/10 0/0 0/0
Bhutan
 Phuntsholing citya 2006 12 12/12 0/0 0/0 0/0 0/0 12/12
Pakistan
 Lahore cityb 2012 39 0/0 0/0 39/39 39/39 0/0 0/0
Indonesia
 Ternate Island 2017 20 19/19 1/1 0/0 0/0 1/1 19/19
 Soppeng Regency, South Sulawesi 2017 12 12/12 0/0 0/0 0/0 0/0 12/12
Total   169 66/66 32/31 71/72 86/86 4/4 79/79
  1. aMosquito DNA samples were obtained from Yanola et al. [10]
  2. bMosquito DNA samples were obtained from Stenhouse et al. [4]
  3. cOne sample was homozygous C/C1534 by AS-PCR, but heterozygous F/C1534 by multiplex PCR and DNA sequencing