A multiplex PCR for detection of knockdown resistance mutations, V1016G and F1534C, in pyrethroid-resistant Aedes aegypti

Table 3 Comparison of genotyping results for V1016G and F1534C mutations from multiplex PCR and AS-PCR

Strain	Year of collection	Total	Multiplex PCR genotyping/AS-PCR genotyping (No. of samples)
			1534			1016
			F/F	F/C	C/C	V/V	V/G	G/G
Thailand
Mae Sariang district, Mae Hong Son^a	2010	15	4/4	5/4	6/7^c	9/9	2/2	4/4
Ranot district, Song Khla^a	2008	10	0/0	3/3	7/7	10/10	0/0	0/0
Ubon Ratchathani city, Ubon Ratchathani^a	2008	9	0/0	1/1	8/8	8/8	1/1	0/0
Myanmar
Yangon city^a	2008	10	4/4	6/6	0/0	10/10	0/0	0/0
Yangon city	2016	32	15/15	16/16	1/1	0/0	0/0	32/32
Cambodia
Battambang city^a	2008	10	0/0	0/0	10/10	10/10	0/0	0/0
Bhutan
Phuntsholing city^a	2006	12	12/12	0/0	0/0	0/0	0/0	12/12
Pakistan
Lahore city^b	2012	39	0/0	0/0	39/39	39/39	0/0	0/0
Indonesia
Ternate Island	2017	20	19/19	1/1	0/0	0/0	1/1	19/19
Soppeng Regency, South Sulawesi	2017	12	12/12	0/0	0/0	0/0	0/0	12/12
Total		169	66/66	32/31	71/72	86/86	4/4	79/79

^aMosquito DNA samples were obtained from Yanola et al. [10]
^bMosquito DNA samples were obtained from Stenhouse et al. [4]
^cOne sample was homozygous C/C1534 by AS-PCR, but heterozygous F/C1534 by multiplex PCR and DNA sequencing

ISSN: 1756-3305