Fig. 4

Analysis of putatively positive colonies. a Agarose gel electrophoresis analysis of amplified PCR products of the inserts on putatively positive prey plasmids. Lane M: DNA 5000 maker; Lanes 1–7: PCR amplification products of the inserts on the seven putatively positive hits. b Confirmation of putative hits. Y2HGold cells were co-transformed with pGBKT7-TaCP and each of the seven putatively positive prey plasmids (numbers 1–7) were plated on QDO/X/A plates; positive interaction was indicated by the presence of blue colonies. Co-transformation with pGADT7-T and pGBKT7-Lam was used as a negative control, while co-transformation with pGADT7-T and pGBKT7-53 was used as a positive control