Fig. 1From: Does evaluation of in vitro microfilarial motility reflect the resistance status of Dirofilaria immitis isolates to macrocyclic lactones?Baseline motility of D. immitis isolate microfilariae. Fifty microfilariae of each isolate were cultured for 24 h at 37 °C and 5% CO2. The larvae in individual wells were recorded for 30 s and the mean movement calculated using the WormAssay software [33]. Each experiment was performed in triplicate, n = 4Back to article page