Fig. 3

Fluorescence observed in transgenic populations of E. tenella. a Transiently transfected sporozoites with MDBK cells at 24 h post-infection. Panel 1: pMIC2-mChe transfected sporozoites; mCherry is seen at the apical end whereas mCitrine is detected in the cytosol. Panel 2: pMCP2-mChe transfected sporozoites; mCherry is seen at the apical end part whereas mCitrine is detected in the cytosol. Panel 3: immunofluorescence (IF) of E. tenella wild type sporozoites probed with anti-EtMIC2 and goat anti-rabbit IgG conjugated to Alexa Fluor 488. The plasmid constructs for each transfection are displayed above each panel. Arrows indicate the anterior end of sporozoites. b Oocysts of stable transgenic populations Et-MIC2-mChe and Et-MCP2-mChe; mCitrine was observed in the cytosol of encysted sporozoites whereas mCherry accumulated in specific patches (location cannot be assigned due the folded position of the sporozoites within the oocyst). c Sporozoites of stable transgenic populations Et-MIC2-mChe and Et-MCP2-mChe incubated with MDBK cells for 24 h; mCherry was observed at apical end whereas mCitrine was detected the cytosol for both populations. Scale-bars: 5 μm