Fig. 3

Phylogenetic tree based on nuclear gene GPI. a Tree inferred with neighbor-joining method and Kimura-2-parameter model. GPI recognizes and differentiates T. cruzi DTUs TcI, TcII, TcIII and TcIV, T. c. marinkellei, T. dionisii and T. rangeli. One allele of TcV and TcVI sequences cluster with TcII, and the other allele clusters with TcIII. Tcbat was placed in the same cluster as TcI. b The tree inferred from maximum likelihood, parsimony and Bayesian inference. T. cruzi, T. c. marinkellei, T. dionisii and T. rangeli are clearly separated from each other. DTUs TcI and TcII are the most genetically divergent. Tcbat, TcI and TcIII fall in the same cluster. One allele of TcV and TcVI clustered with TcII, and the other allele clustered with the group comprising TcI, TcIII and Tcbat. The numbers at the nodes correspond to ML, MP and BI support values, respectively (only values >60 are shown). The scale-bar shows the number of nucleotide substitutions per site