Fig. 3

Src inhibitor-1 inhibits EhSrc protein activation of E. histolytica in silico and in vivo. a Predicted binding site of Src inhibitor-1 in the EhSrc protein of E. histolytica. b Confocal microscopy analysis of trophozoites with or without Src inhibitor-1 (30 μM) treatment. Actin was stained with rhodamine-phalloidin (red) (1/25), the nucleus was identified with DAPI 1/300 (blue) and p-Src (green) was detected with an anti-p-Src antibody (1/50). c Detection by western blot of phosphorylated tyrosine (p-Tyr), actin (α-actin) and EhSrc in EhSrc immunoprecipitated complex from treated and non-treated trophozoites with anti-phospho-tyrosine (1/1000), anti-actin (1/5000), and anti-EhSrc (1/5000) antibodies. d The kinase activity of DMSO- and Src inhibitor-1-treated trophozoites was determined using a Pro-Fluor® Src-Family kinase assays (Promega, Fitchburg, WI, USA) according to manufacturer’s instructions; the kinase activity from DMSO-treated trophozoites was defined as 100%. Values for each group represent mean ± SEM results from three independent experiments