Fig. 4

EhSrc interacts with actin during actin cytoskeleton reorganization. a Confocal microscopy analysis of trophozoites adhered to FN; actin was stained with rhodamine-phalloidin (red) (1/25), and Src (green) was detected with EhSrc antibody (1/250) and FITC-labeled goat anti- rabbit IgG (1/100). The white arrows in the merged image indicate the colocalization sites. At the bottom of panel (b) and to the right of panel (c), histograms of green and red intensities corresponding to selected areas (white boxes in confocal images) are shown. Areas of colocalization are marked with yellow arrows; areas where no colocalization was found are marked with green arrows. In each panel (b and c), the dispersion values indicating the results of the colocalization analyzes​​ are shown. d Predicted binding site of EhSrc and actin of E. histolytica. Image processing was performed with the Zen Blue software (v. 2012); the Pearson correlation coefficient was calculated with the SPSS software (v. 24). Abbreviation: R, Pearson correlation coefficient. Scale-bars: 10 μm