Skip to main content

Table 2 Endosymbiont PCR screening and DNA sequencing of Culicoides collected at natural habitats and livestock premises in Spain. Tests results are segregated horizontally by group of Culicoides spp. and NUTS2 regions

From: First detection of Wolbachia-infected Culicoides (Diptera: Ceratopogonidae) in Europe: Wolbachia and Cardinium infection across Culicoides communities revealed in Spain

Group NUTS2 Wolbachia Cardinium Year collection
Livestock premise Natural habitat Livestock premise Natural habitat
W+ W- W+ W- C+ C- C+ C- 2009 2010 2011 2012
IMI   1 115 5 135 0 108 0 140     
Andalucía 1 (1_Seq) 47 5 (4_Seq) 95 0 48 0 100 +   +  
Castilla Mancha 0 50 0 40 0 50 0 40 + +   
Catalunya 0 18 0 10 +    
OBS   0 162 2 301 1 151 1 300     
Andalucía 0 50 0 80 0 50 0 80   + +  
Asturias 0 10 2 (2_Seq) 101 0 10 1 (1_Seq) 100 + +   
Castilla Mancha 0 70 0 70   +   
Catalunya 0 102 0 50 1 (1_Seq) 91 0 50 +   + +
PUL   0 5 56 248 0 3 0 300     
Andalucía 47 (8_Seq) 54 0 100 +    
Asturias 2 (1_Seq) 98 0 100 + +   
Castilla Mancha 7 (5_Seq) 44 0 50   + +  
Catalunya 0 5 0 52 0 3 0 50 +   +  
Other   3 39 0 8 9 31 1 5     
Andalucía 0 2 0 2 +    
Asturias 0 3 0 2 + +   
Castilla Mancha 0 3 1 1 + +   
Catalunya 3 (2_Seq) 39 9 (6_Seq) 31 + +   
Total   4 321 63 692 10 293 2 745     
  1. Abbreviations: W+ no. of Wolbachia-positive (PCR) tests; W- no. of Wolbachia-negative (PCR) tests; C+ no. of Cardinium-positive (PCR) tests; C- no. of Cardinium-negative (PCR) tests. The number of Culicoides for which endosymbiont DNA sequences were obtained are shown in parentheses. Definition of species groups: IMI, C. imicola; OBS, C. obsoletus (s.l.); PUL, C. pulicaris (s.l.); Other; other Culicoides spp