Table 2 Endosymbiont PCR screening and DNA sequencing of Culicoides collected at natural habitats and livestock premises in Spain. Tests results are segregated horizontally by group of Culicoides spp. and NUTS2 regions
Group | NUTS2 | Wolbachia | Cardinium | Year collection | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Livestock premise | Natural habitat | Livestock premise | Natural habitat | ||||||||||
W+ | W- | W+ | W- | C+ | C- | C+ | C- | 2009 | 2010 | 2011 | 2012 | ||
IMI | 1 | 115 | 5 | 135 | 0 | 108 | 0 | 140 | |||||
Andalucía | 1 (1_Seq) | 47 | 5 (4_Seq) | 95 | 0 | 48 | 0 | 100 | + | + | |||
Castilla Mancha | 0 | 50 | 0 | 40 | 0 | 50 | 0 | 40 | + | + | |||
Catalunya | 0 | 18 | – | – | 0 | 10 | – | – | + | ||||
OBS | 0 | 162 | 2 | 301 | 1 | 151 | 1 | 300 | |||||
Andalucía | 0 | 50 | 0 | 80 | 0 | 50 | 0 | 80 | + | + | |||
Asturias | 0 | 10 | 2 (2_Seq) | 101 | 0 | 10 | 1 (1_Seq) | 100 | + | + | |||
Castilla Mancha | – | – | 0 | 70 | – | – | 0 | 70 | + | ||||
Catalunya | 0 | 102 | 0 | 50 | 1 (1_Seq) | 91 | 0 | 50 | + | + | + | ||
PUL | 0 | 5 | 56 | 248 | 0 | 3 | 0 | 300 | |||||
Andalucía | – | – | 47 (8_Seq) | 54 | – | – | 0 | 100 | + | ||||
Asturias | – | – | 2 (1_Seq) | 98 | – | – | 0 | 100 | + | + | |||
Castilla Mancha | – | – | 7 (5_Seq) | 44 | – | – | 0 | 50 | + | + | |||
Catalunya | 0 | 5 | 0 | 52 | 0 | 3 | 0 | 50 | + | + | |||
Other | 3 | 39 | 0 | 8 | 9 | 31 | 1 | 5 | |||||
Andalucía | – | – | 0 | 2 | – | – | 0 | 2 | + | ||||
Asturias | – | – | 0 | 3 | – | – | 0 | 2 | + | + | |||
Castilla Mancha | – | – | 0 | 3 | – | – | 1 | 1 | + | + | |||
Catalunya | 3 (2_Seq) | 39 | – | – | 9 (6_Seq) | 31 | – | – | + | + | |||
Total | 4 | 321 | 63 | 692 | 10 | 293 | 2 | 745 | |||||