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Table 1 pCS20 Sol1TqM qPCR sensitivity and specificity test on Ehrlichia ruminantium strains and other tick-borne pathogens

From: Efficient high-throughput molecular method to detect Ehrlichia ruminantium in ticks

Name Origin Geographical origin Nested pCS20 PCR Sol1TqM QPCR Reference
E. ruminantium strains
 Blonde CC p8 Guadeloupe + + [31]
 Gardel CC p48 + + [29]
 Sara 455 CC p10 Burkina Faso + + [8]
 Bekuy 255 CC p9 + + [31]
 Bankouma 421 CC p15 + + [8]
 Banankeledaga CC p1 + w+ [8]
 Lamba 479 CC p16 + + [31]
 Cameroun CC p9 Cameroon + + [31]
 Pokoase 412 CC p10 Ghana + + [31]
 Senegal CC p60 Senegal + + [31]
 Umbaneim B Sudan + + [31]
 Mara CC p1 South Africa + + [31]
 Welgevonden CC p12 + + [29]
 Lutale CC p6 Zambia + + [31]
 Sankat 430 CC p16 Ghana + + [32]
 Umpala CC p6 Mozambique + + [31]
 MAT2-17MH1 T + w+ [33]
 MAT2-26MH1   + + [33]
 MAT2-32MH2   + w+ [33]
 CHOB4MH2   + w + [33]
 CHOB25MH1   + w+ [33]
 CHA2-32MH1   + w+ [33]
 KNP51MH1 T South Africa + w+ [33]
 KNPC15MH1   w+ w+ [33]
 KNPC2MH1   + + [33]
 KNPC2MH2   + + [33]
Other tick-borne pathogens
A. marginale   Argentina  
A. phagocytophilum CC USA  
A. platys (E. platys) CC  
B. bovis   Argentina  
B. bigemina   
E. canis CC USA [34]
E. muris CC [35]
 PME 160055 T +  
 PME 160178   
 PME 160277   +  
 PME 160359   
 PME 160366   +  
 PME 160491   +  
R. felis CC [36]
R. parkeri CC [37]
9 uninfected A. variegatum T Guadeloupe  
  1. E. ruminantium was detected from A. variegatum and A. hebraeum ticks while PME was detected from A. americanum ticks
  2. Abbreviations: T ticks, B blood, CC p cell culture passage, + positive, negative, w + weak positive