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Table 2 Limit of detection of pCS20 Sol1TqM qPCR, Sol1SG qPCR and pCS20 nested PCR

From: Efficient high-throughput molecular method to detect Ehrlichia ruminantium in ticks

DNA
E. ruminantium strain Gardel (copies/reaction)a
Sol1TqM qPCR
(Th = 55 °C)
Ct ± SDb
Sol1SG qPCR
(Th = 51 °C)
Ct ± SDb
pCS20 nested PCR signalc
3.106 17.0 ± 0.3 13.6 ± 0.6 +
3.105 20.3 ± 0.1 16.8 ± 0.4 +
3.104 23.6 ± 0.0 20.0 ± 0.5 +
3.103 27.1 ± 0.6 23.5 ± 0.4 +
3.102 31.0 ± 0.5 26.9 ± 1.0 +
30 34.2 ± 0.3 30.5 ± 1.3 w+
3 36.7d 34.0 ± 0.7
NTC Undet 35.0 ± 1.1
  1. aBacterial load used for qPCR: the nested PCR samples were amplified from 1 μl of DNA, containing half the quantity from 1.5 ×106 to 1.5 copies/reaction
  2. bThe average Ct value is indicated for each dilution (bacteria copy number) and standard deviation was derived from 3 replicates
  3. cConventional PCR done in duplicate
  4. dTested once
  5. Abbreviations: Th temperature of hybridization, w + weak positive, NTC non-template control, Undet undetermined, SD standard deviation