Table 3 Relative sensitivity and specificity of automated DNA extraction compared with manual DNA extraction based on screening of samples tested either by nested pCS20 PCR or Sol1^TqM qPCR. Results (positive and negative E. ruminantium samples, Se, Sp and Acc of the automated extraction method) obtained on tick lysates spiked with E. ruminantium serial dilutions (n = 17) and experimentally infected ticks (n = 30) extracted in parallel by manual and automatic extraction and tested either by nested PCR or pCS20 Sol1^TqM qPCR are shown. The kappa test was 72%

From: Efficient high-throughput molecular method to detect Ehrlichia ruminantium in ticks