Table 4 Relative sensitivity and specificity of pCS20 Sol1TqM qPCR compared with the pCS20 nested PCR and MLST, which were combined as the reference method. Results obtained from 60 field-collected ticks are shown. The kappa test was 60%
From: Efficient high-throughput molecular method to detect Ehrlichia ruminantium in ticks
Nested PCR + MLST | Total | Se (%) | Sp (%) | Ac (%) | |||
|---|---|---|---|---|---|---|---|
+ | – | ||||||
Sol1TqM qPCR | + | 25 | 4 | 29 | 75.8 | 85.2 | 80.0 |
– | 8 | 23 | 31 | ||||
Total | 33 | 27 | 60 | ||||