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Table 4 Relative sensitivity and specificity of pCS20 Sol1TqM qPCR compared with the pCS20 nested PCR and MLST, which were combined as the reference method. Results obtained from 60 field-collected ticks are shown. The kappa test was 60%

From: Efficient high-throughput molecular method to detect Ehrlichia ruminantium in ticks

   Nested PCR + MLST Total Se (%) Sp (%) Ac (%)
   +     
Sol1TqM qPCR + 25 4 29 75.8 85.2 80.0
8 23 31    
Total 33 27 60    
  1. Se relative sensitivity, Sp relative specificity, Ac accuracy