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Table 5 Relative sensitivity and specificity of automated extraction + pCS20 Sol1TqM qPCR compared with manual extraction + pCS20 nested PCR. Results obtained on 17 E. ruminantium serial dilutions and 30 experimentally infected ticks. The kappa test for this analysis was 49%

From: Efficient high-throughput molecular method to detect Ehrlichia ruminantium in ticks

   Manual extraction + pCS20 nested PCR Total Se (%) Sp (%) Ac (%)
   +     
Automated extraction +Sol1TqM qPCR + 16 7 23 76.2 73.1 74.5
5 19 24    
Total 21 26 47    
  1. Se relative sensitivity, Sp relative specificity, Ac accuracy