Table 5 Relative sensitivity and specificity of automated extraction + pCS20 Sol1TqM qPCR compared with manual extraction + pCS20 nested PCR. Results obtained on 17 E. ruminantium serial dilutions and 30 experimentally infected ticks. The kappa test for this analysis was 49%
From: Efficient high-throughput molecular method to detect Ehrlichia ruminantium in ticks
Manual extraction + pCS20 nested PCR | Total | Se (%) | Sp (%) | Ac (%) | |||
|---|---|---|---|---|---|---|---|
+ | – | ||||||
Automated extraction +Sol1TqM qPCR | + | 16 | 7 | 23 | 76.2 | 73.1 | 74.5 |
– | 5 | 19 | 24 | ||||
Total | 21 | 26 | 47 | ||||