Fig. 1

Sequence analysis of CsPK. An alignment of the protein sequence of CsPK with those of PKs from other organisms is shown. Clonorchis sinensis (C.s, GAA54498.1), Schistosoma japonicum (S.j, AAW27129.1), Eimeria tenella (E.t, AAC02529.1), Toxoplasma gondii (T.g, BAB47171.1), Plasmodium falciparum (P.f, CAD50538.1), Leishmania mexicana (L.m, CAA52898.2), Trypanosoma brucei (T.b, P30615.1), Trypanosoma cruzi (T.c, EKG02834.1), Mastigamoeba balamuthi (M.b, AAK94944.1), Cryptosporidium parvum (C.p, 4DRS_A), Homo sapiens (H.s, AAA60104.1), Mus musculus (M.m, NP_001093249.1), and Rattus norvegicus (R.n, AAA41880.1). The 3D–domains (N/A/B/C) are marked with vertical lines. The 22 predicted B cell linear epitopes with more trustworthiness are indicated by the black lines above the alignment. The rectangle indicates the PK active site signature. The triangles and open squares indicate the PEP and ADP binding sites, respectively. The binding sites of the sugar, 1-phosphate and 6-phosphate moieties of the allosteric activator fructose-1,6-bisphosphate (F16BP) are indicated by closed squares, open circles and closed circles, respectively. The black arrows and asterisks indicate monovalent cation and divalent cation binding sites, respectively