Fig. 3From: Sequence analysis and characterization of pyruvate kinase from Clonorchis sinensis, a 53.1-kDa homopentamer, implicated immune protective efficacy against clonorchiasisExpression and apparent Mr. of rCsPK. Proteins were visualized by Coomassie Blue staining. Lane M contains protein molecular weight markers. a Expression and purification of rCsPK. Lysate of E. coli transformed with pET-28a(+)-CsPK without induction (Lane 1) and with induction (Lane 2); supernatant (Lane 3) and precipitate (Lane 4) of lysate of E. coli with pET-28a(+)-CsPK with induction; and purified rCsPK (Lane 5). b In Lane M, the protein bands with known Mr. (in descending order) are thyroglobulin (669 kDa), ferritin (440 kDa), catalase (232 kDa), lactate dehydrogenase (140 kDa), and BSA (67 kDa). Lane 1, Freshly purified rCsPK; Lane 2, rCsPK stored for 4 weeks at -80 °C with 4 cycles of freezing and thawingBack to article page