Fig. 6

Immunolocalization of CsPK in C. sinensis. Mouse anti-rCsPK sera and Cy3-conjugated goat anti-mouse IgG were used as the primary and secondary antibodies, respectively. Preimmune mouse serum was used as the primary antibody for the negative controls. Panels (c), (d), (e), and (f) show negative controls. Panels (b), (d), (f), and h are fluorescence microscopic images; the same areas of the samples photographed under white light are shown in panels (a), (c), (e), and g with scale-bars. Panel (b), localization of CsPk in adults; panel h, localization of CsPk in metacercariae. Abbreviations: tg, tegument; e, egg; v, vitellarium; os, oral sucker; vs, ventral sucker; g, genital pore; s, seminal receptacle; i, intestine; ts, testicle; u, uterus;o, ovary; p, pharynx. Scale-bars: a-d, 100 μm; e-h, 10 μm