Fig. 8

Rat anti-rCsPK sera inhibits C. sinensis adult subsistence in vitro. The median subsistence times of C. sinensis adults in the blank control group, the 1:40 preimmune serum group, the 1:80 preimmune serum group, the 1:160 preimmune serum group, the 1:40 anti-rCsPK serum group, the 1:80 anti-rCsPK serum group, and the 1:160 anti-rCsPK serum group were 15, 8, 8, 9, 2, 3 and 4 days, respectively. No significant difference in the rate of survival of the preimmune serum groups was observed at any serum dilution (1:40 preimmune serum group vs 1:80 preimmune serum group: χ ² = 0.01289, df = 1, P = 0.9096; 1:80 preimmune serum group vs 1:160 preimmune serum group: χ ² = 0.09872, df = 1, P = 0.7534; 1:40 preimmune serum group vs 1:160 preimmune serum group: χ ² = 0.1657, df = 1, P = 0.6839). There were significant differences among the other groups in the rate of survival (1:40 anti-rCsPK serum group vs 1:80 anti-rCsPK serum group: χ ² = 8.058, df = 1, P = 0.0045; 1:80 anti-rCsPK serum group vs 1:160 anti-rCsPK serum group: χ ² = 8.092, df = 1, P = 0.0044; blank control group vs 1:40 preimmune serum group: χ ² = 16.15, df = 1, P < 0.0001; blank control group vs 1:80 preimmune serum group: χ ² = 15.54, df = 1, P < 0.0001; blank control group vs 1:160 preimmune serum group: χ ² = 11.39, df = 1, P = 0.0007)