Fig. 4

NGS results for 10 I. ricinus tick samples. Two samples positive in one or more pathogen screening PCR (a) and 8 samples negative in all screening PCRs (b) were analyzed. Whole genome amplified samples were sequenced on an Ion S5™, Kraken was used for taxonomic profiling of trimmed reads, and species with low read support were filtered out. Species pathogenic for humans, i.e. R. helvetica (R.h.), A. phagocytophilum (A.p.), B. afzelii (B.a.), as well as the tick endosymbiont "Candidatus Midichloria mitochondrii" (C.M.m.), are represented individually. The remaining species are grouped in Pseudomonas spp. (P.spp.), other residents of soil and water, commensals, human microbiota, and plant associated bacteria. The bars indicate the percentages of reads assigned to the respective species or groups in a logarithmic scale