Species | Primer sequences (5'–3') | Amplicon length (bp) | Amplicon position (reference sequence) | Annealing T (°C) | Reference |
---|---|---|---|---|---|
Fist-step PCR | Â | Â | Â | Â | |
  Borrelia spp. | locus: 5S-23S intergenic spacer (forward: GAG TTC GCG GGA GAG TAG GTT ATT; reverse: TCA GGG TAC TTA GAT GGT TCA CTT CC | 420 | 3063–3483 (JX564636.1) | 64 | [111] |
  Babesia spp. | locus: 18S ribosomal RNA gene (forward: GTC TTG TAA TTG GAA TGA TGG; reverse: TAG TTT ATG GTT AGG ACT ACG) | 489 | 466–955 (AJ439713) | 58 | [112] |
  Rickettsia spp. | locus: 23S–5S intergenic spacer (forward: GAT AGG TCR GRT GTG GAA GCA C; reverse: TCG GGA YGG GAT CGT GTG TTT C) | 388 | 1–388 (AY125012) | 68 | [113] |
 Ticks | locus: cytochrome c oxidase (forward: ACW AAY CAY AAA GAC ATT GGA AC; reverse: WGG ATG CCC RAA RAA TCA AAA T) | 704 | 1242–1946 (KF197132) | 48 | [114] |
Second-step PCR | Â | Â | Â | Â | |
  Borrelia spp. | locus: 23S-5S intergenic spacer (forward: GGA GAG TAG GTT ATT GCC AG; reverse: GGT TCA CTT CCC CTG GTA TC) | 396 | 3073–3468 (JX56436.1) | 64 | – |
  Babesia spp. | locus: 18S ribosomal RNA gene (forward: GTA ATT GGA ATG ATG GTG AC; reverse: GTT AGG ACT ACG ACG GAA TC) | 475 | 471–946 (AJ439713) | 58 | – |
  Rickettsia spp. | locus: 23S-5S intergenic spacer (forward: CAG TAA TGT GTG TAG CTA AC; reverse: ATC GTG TGT TTC ACT CAT GC) | 356 | 22–378 (AY125012) | 58 | – |
 Ticks | locus: cytochrome c oxidase (forward: AYC AYA AAG ACA TTG GAA CWA T; reverse: GCC CRA ARA ATC AAA ATA RAT G) | 686 | 1245–1933 (KF197132) | 48 | – |